Part:BBa_P1016:Design

ccdB cassette without ccdA-

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 323
  

Design Notes

BBa_P1016 differs from BBa_P1010 in that the DNA encoding the mutated antitoxin CcdA protein (ccdA-) has been deleted since it is extraneous to the function of this part as a positive selection marker.

BBa_P1016 differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part (thus the coding region of ccdB is similar to commercially available versions of ccdB). It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.

Source

This part was designed based on BBa_P1010 but it lacks ccdA-. It was synthesized via direct synthesis by Codon Devices, Inc. in Cambridge, MA as a part of a larger part BBa_I51020.

References

<biblio>

1. Bernard-J-Mol-Biol-1992 pmid=1324324
2. Miki-J-Mol-Biol-1992 pmid=1316444
3. Bernard-Gene-1994 pmid=7926841
4. Bernard-Gene-1995 pmid=7557407
5. Bernard-Biotechniques-1996 pmid=8862819

</biblio>

• US Patent Number 5,910,438 "Cloning and/or sequencing vector" Bernard P, Gabant P, University Libre de Bruxelles, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
• US Patent Number 6,180,407 B1 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
• US Patent Number 7,176,029 B2 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]